Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating targeting expression of genes. It very important to normalize according stably expressed housekeeping genes in order facilitating gene studies acquire exact meaningful results. The purpose this study was identify validate six (GADPH, RPS18, α-TUB, EF1α, ArgK ACTB) adults cockroach species Blaptica dubia employing five different algorithms (geNorm, Bestkeeper, Normfinder, ΔCt RefFinder) assess putative stability. Our also showed that the geNorm, Normfinder RefFinder identified GADPH as most stable B. adults. Additioanlly, RPS18 suggested by GeNorm BestKeeeper. ACTB has been shown be far least all algorithms. In addition, since there are few validation reference cockroaches literature, it considered would beneficial increase number related with RT-qPCR on under biotic abiotic conditions cockroaches.